pGolf-ISH
(Plasmid #15853)

• Depositing Lab: Peter Mombaerts
• Publication: Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-88.

Backbone

• Vector backbone: pGEM-T Easy
• Backbone manufacturer: promega
• Backbone size w/o insert (bp): 3015
• Vector type: cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Guanine Nucleotide Binding Protein, Alpha Stimulating, Olfactory type
• Alt name: Golf
• Species: M. musculus (mouse)
• Insert Size (bp): 1130
• Mutation: F199S
• GenBank ID: NM_010307
• Entrez Gene: Gnal (a.k.a. 2610011C15Rik, 9630020G10Rik, Galphaolf, Gna10, Golf)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13, T7
• 3′ sequencing primer: M13 reverse, SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was used to prepare RNA probes for in situ hybridization (ISH) and has not been used for functional studies of Gnal. It is not known whether the F199S mutation may affect function of Gnal. Moreover, be advised that the plasmid does not contain the full coding region of Gnal.