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Addgene

pZA5
(Plasmid #158483)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158483 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR8/GW/D-TOPO
  • Backbone manufacturer
    https://www.thermofisher.com/order/catalog/product/K250020#/K250024
  • Vector type
    Golden Gate Entry Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZAR1
  • Species
    N. benthamiana
  • Mutation
    STOP codon

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZA5 was a gift from Sophien Kamoun (Addgene plasmid # 158483 ; http://n2t.net/addgene:158483 ; RRID:Addgene_158483)

  • For your References section:

    A vector system for fast-forward studies of the HOPZ-ACTIVATED RESISTANCE1 (ZAR1) resistosome in the model plant Nicotiana benthamiana. Harant A, Pai H, Sakai T, Kamoun S, Adachi H. Plant Physiol. 2022 Jan 20;188(1):70-80. doi: 10.1093/plphys/kiab471. 10.1093/plphys/kiab471 PubMed 34633454
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