pZA1
(Plasmid
#158479)
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PurposeEntry clone for Golden Gate assembly. Golden Gate compatible wild-type A. thaliana ZAR1 with STOP codon
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158479 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR8/GW/D-TOPO
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Backbone manufacturerhttps://www.thermofisher.com/order/catalog/product/K250020#/K250020
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Vector typeGolden Gate Entry Vector
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZAR1
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SpeciesA. thaliana (mustard weed)
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MutationSTOP codon
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Entrez GeneZAR1 (a.k.a. HOPZ-ACTIVATED RESISTANCE 1, ZAR1)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZA1 was a gift from Sophien Kamoun (Addgene plasmid # 158479 ; http://n2t.net/addgene:158479 ; RRID:Addgene_158479) -
For your References section:
A vector system for fast-forward studies of the HOPZ-ACTIVATED RESISTANCE1 (ZAR1) resistosome in the model plant Nicotiana benthamiana. Harant A, Pai H, Sakai T, Kamoun S, Adachi H. Plant Physiol. 2022 Jan 20;188(1):70-80. doi: 10.1093/plphys/kiab471. 10.1093/plphys/kiab471 PubMed 34633454