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Addgene

pMH-25
(Plasmid #158436)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158436 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD33
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    JPA605 (parent strain RP437)
  • Growth instructions
    Plasmid was deposited to Addgene in a custom bacterial strain, JPA605 (parent strain RP437). However, the plasmid may easily be stored and expressed in DH5alpha.
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    MotA
  • Species
    E. coli
  • Insert Size (bp)
    888

Gene/Insert 2

  • Gene/Insert name
    MotB
  • Species
    E. coli
  • Insert Size (bp)
    927
  • Tag / Fusion Protein
    • yPET - 3 x EAAAK linker (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH-25 was a gift from Francesco Pedaci (Addgene plasmid # 158436 ; http://n2t.net/addgene:158436 ; RRID:Addgene_158436)

  • For your References section:

    Impact of fluorescent protein fusions on the bacterial flagellar motor. Heo M, Nord AL, Chamousset D, van Rijn E, Beaumont HJE, Pedaci F. Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.1038/s41598-017-11241-w. 10.1038/s41598-017-11241-w PubMed 28974721
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