pYPQ141-ZmUbi-RZ-Aa3.8.5
(Plasmid
#158407)
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Purpose(Empty Backbone) Express single gRNA with Aa3.8.5 scaffold (with four MS2 binding sites) under ZmUbi promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158407 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYPQ141D
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Vector typePlant Expression, CRISPR
- Promoter Maize ubiquitin 1
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYPQ141-ZmUbi-RZ-Aa3.8.5 was a gift from Yiping Qi (Addgene plasmid # 158407 ; http://n2t.net/addgene:158407 ; RRID:Addgene_158407) -
For your References section:
CRISPR-Act3.0 for highly efficient multiplexed gene activation in plants. Pan C, Wu X, Markel K, Malzahn AA, Kundagrami N, Sretenovic S, Zhang Y, Cheng Y, Shih PM, Qi Y. Nat Plants. 2021 Jul;7(7):942-953. doi: 10.1038/s41477-021-00953-7. Epub 2021 Jun 24. 10.1038/s41477-021-00953-7 PubMed 34168320
