pYPQ143-ZmUbi-tRNA
(Plasmid
#158400)
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Purpose(Empty Backbone) Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ133-tRNA2.0; assembly of 3 gRNAs
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158400 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYPQ141D
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Vector typePlant Expression, CRISPR
- Promoter Maize ubiquitin 1
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYPQ143-ZmUbi-tRNA was a gift from Yiping Qi (Addgene plasmid # 158400 ; http://n2t.net/addgene:158400 ; RRID:Addgene_158400) -
For your References section:
CRISPR-Act3.0 for highly efficient multiplexed gene activation in plants. Pan C, Wu X, Markel K, Malzahn AA, Kundagrami N, Sretenovic S, Zhang Y, Cheng Y, Shih PM, Qi Y. Nat Plants. 2021 Jul;7(7):942-953. doi: 10.1038/s41477-021-00953-7. Epub 2021 Jun 24. 10.1038/s41477-021-00953-7 PubMed 34168320