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pEMS2279
(Plasmid #158399)

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Item Catalog # Description Quantity Price (USD)
Plasmid 158399 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    GenScript
  • Total vector size (bp) 4731
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ple349
  • Alt name
    PDE6H MiniPromoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2021
  • Entrez Gene
    PDE6H (a.k.a. ACHM6, RCD3)
  • Promoter Ple349

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site FseI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer M13-fwd
  • 3′ sequencing primer M13-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid commercially produced by Genscript . Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid, including the MiniPromoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS2279 was a gift from Elizabeth Simpson (Addgene plasmid # 158399 ; http://n2t.net/addgene:158399 ; RRID:Addgene_158399)

  • For your References section:

    Human MiniPromoters for ocular-rAAV expression in ON bipolar, cone, corneal, endothelial, Muller glial, and PAX6 cells. Korecki AJ, Cueva-Vargas JL, Fornes O, Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Zhou M, Wasserman WW, Di Polo A, Simpson EM. Gene Ther. 2021 Feb 2. pii: 10.1038/s41434-021-00227-z. doi: 10.1038/s41434-021-00227-z. 10.1038/s41434-021-00227-z PubMed 33531684
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