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Purpose(Empty Backbone) Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158393 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHD1
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Backbone manufacturerCermak et al. 2011
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Vector typePlant Expression, CRISPR
- Promoter Without promoter
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYPQ131-tRNA2.0 was a gift from Yiping Qi (Addgene plasmid # 158393 ; http://n2t.net/addgene:158393 ; RRID:Addgene_158393) -
For your References section:
CRISPR-Act3.0 for highly efficient multiplexed gene activation in plants. Pan C, Wu X, Markel K, Malzahn AA, Kundagrami N, Sretenovic S, Zhang Y, Cheng Y, Shih PM, Qi Y. Nat Plants. 2021 Jul;7(7):942-953. doi: 10.1038/s41477-021-00953-7. Epub 2021 Jun 24. 10.1038/s41477-021-00953-7 PubMed 34168320
