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Addgene

MAC-SARS-CoV-2 NSP10
(Plasmid #158361)

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Item Catalog # Description Quantity Price (USD)
Plasmid 158361 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    MAC-tag-C (#108077)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 NSP10
  • Species
    Other
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Tag / Fusion Protein
    • MAC-tag (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAC-SARS-CoV-2 NSP10 was a gift from Markku Varjosalo (Addgene plasmid # 158361 ; http://n2t.net/addgene:158361 ; RRID:Addgene_158361)

  • For your References section:

    Combined proximity labeling and affinity purification-mass spectrometry workflow for mapping and visualizing protein interaction networks. Liu X, Salokas K, Weldatsadik RG, Gawriyski L, Varjosalo M. Nat Protoc. 2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 10.1038/s41596-020-0365-x. 10.1038/s41596-020-0365-x PubMed 32778839
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