GAL FLAG46QDProGFP p306
(Plasmid
#15832)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15832 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep306 Gal
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehtt 46Q Delta Pro
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Alt namehtt 46Q
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SpeciesH. sapiens (human)
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MutationProline-rich region removed
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GenBank IDNM_002111
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Entrez GeneHTT (a.k.a. HD, IT15, LOMARS)
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Tags
/ Fusion Proteins
- FLAG (N terminal on insert)
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Gal1 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Lindquist lab number: 8359.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GAL FLAG46QDProGFP p306 was a gift from Susan Lindquist (Addgene plasmid # 15832 ; http://n2t.net/addgene:15832 ; RRID:Addgene_15832) -
For your References section:
Flanking sequences profoundly alter polyglutamine toxicity in yeast. Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S. Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50. 10.1073/pnas.0604547103 PubMed 16832050