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Addgene

pSL21-VEX
(Plasmid #158230)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158230 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSL21-VEX
  • Backbone size (bp) 6619
  • Vector type
    Mammalian Expression, Retroviral, CRISPR
  • Promoter U6 promoter for crRNA expression and EFS promoterfor VEX expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSL21-VEX was a gift from Junwei Shi (Addgene plasmid # 158230 ; http://n2t.net/addgene:158230 ; RRID:Addgene_158230)

  • For your References section:

    In vivo CD8(+) T cell CRISPR screening reveals control by Fli1 in infection and cancer. Chen Z, Arai E, Khan O, Zhang Z, Ngiow SF, He Y, Huang H, Manne S, Cao Z, Baxter AE, Cai Z, Freilich E, Ali MA, Giles JR, Wu JE, Greenplate AR, Hakeem MA, Chen Q, Kurachi M, Nzingha K, Ekshyyan V, Mathew D, Wen Z, Speck NA, Battle A, Berger SL, Wherry EJ, Shi J. Cell. 2021 Mar 4;184(5):1262-1280.e22. doi: 10.1016/j.cell.2021.02.019. Epub 2021 Feb 25. 10.1016/j.cell.2021.02.019 PubMed 33636129
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