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Addgene

Human J-chain
(Plasmid #158215)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158215 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    JCHAIN
  • Species
    H. sapiens (human)
  • Entrez Gene
    JCHAIN (a.k.a. IGCJ, IGJ, JCH)
  • Tag / Fusion Protein
    • 8×his (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human J-chain was a gift from Junyu Xiao (Addgene plasmid # 158215 ; http://n2t.net/addgene:158215 ; RRID:Addgene_158215)
  • For your References section:

    Structural insights into immunoglobulin M. Li Y, Wang G, Li N, Wang Y, Zhu Q, Chu H, Wu W, Tan Y, Yu F, Su XD, Gao N, Xiao J. Science. 2020 Feb 28;367(6481):1014-1017. doi: 10.1126/science.aaz5425. Epub 2020 Feb 6. 10.1126/science.aaz5425 PubMed 32029689