pW5Y_Apr
(Plasmid
#158211)
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Purpose(Empty Backbone) BAC-based, lacUV5 regulon and Cre recombinase in loxP and lox5171 sites
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158211 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBeloBAC11
Growth in Bacteria
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Bacterial Resistance(s)Apramycin, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Resource Information
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Supplemental Documents
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pW5Y_Apr was a gift from Yasuo Yoshikuni (Addgene plasmid # 158211 ; http://n2t.net/addgene:158211 ; RRID:Addgene_158211) -
For your References section:
CRAGE enables rapid activation of biosynthetic gene clusters in undomesticated bacteria. Wang G, Zhao Z, Ke J, Engel Y, Shi YM, Robinson D, Bingol K, Zhang Z, Bowen B, Louie K, Wang B, Evans R, Miyamoto Y, Cheng K, Kosina S, De Raad M, Silva L, Luhrs A, Lubbe A, Hoyt DW, Francavilla C, Otani H, Deutsch S, Washton NM, Rubin EM, Mouncey NJ, Visel A, Northen T, Cheng JF, Bode HB, Yoshikuni Y. Nat Microbiol. 2019 Dec;4(12):2498-2510. doi: 10.1038/s41564-019-0573-8. Epub 2019 Oct 14. 10.1038/s41564-019-0573-8 PubMed 31611640