pcDNA3-DN-hCUL3-FLAG
(Plasmid #15820)

• Depositing Lab: Wade Harper
• Publication: Jin et al Methods Enzymol. 2005 . 399():287-309.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cullin3
• Alt name: Cullin 3
• Alt name: Cul3
• Species: H. sapiens (human)
• Insert Size (bp): 1250
• Mutation: Dominant negative: N418
• Entrez Gene: CUL3 (a.k.a. CUL-3, NEDAUS, PHA2E)
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII/BamHI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

C-terminal truncation of hCul3: amino acids 1-418.