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Addgene

p33Fn
(Plasmid #158155)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158155 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRGEB33
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Mutation
    Rice codon optimized

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The rice codon optimized FnCas12a was synthesized at GenScript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p33Fn was a gift from Kabin Xie (Addgene plasmid # 158155 ; http://n2t.net/addgene:158155 ; RRID:Addgene_158155)

  • For your References section:

    Engineering Introns to Express RNA Guides for Cas9- and Cpf1-Mediated Multiplex Genome Editing. Ding D, Chen K, Chen Y, Li H, Xie K. Mol Plant. 2018 Apr 2;11(4):542-552. doi: 10.1016/j.molp.2018.02.005. Epub 2018 Feb 17. 10.1016/j.molp.2018.02.005 PubMed 29462720
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