p32Lb
(Plasmid
#158154)
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Purpose(Empty Backbone) Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and FnCpf1, respectively. For plant genome editing.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158154 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRGEB32
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Vector typePlant Expression, CRISPR
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byLbCas12a gene originates from plasmid pcDNA3.1-hLbCpf1
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p32Lb was a gift from Kabin Xie (Addgene plasmid # 158154 ; http://n2t.net/addgene:158154 ; RRID:Addgene_158154) -
For your References section:
Engineering Introns to Express RNA Guides for Cas9- and Cpf1-Mediated Multiplex Genome Editing. Ding D, Chen K, Chen Y, Li H, Xie K. Mol Plant. 2018 Apr 2;11(4):542-552. doi: 10.1016/j.molp.2018.02.005. Epub 2018 Feb 17. 10.1016/j.molp.2018.02.005 PubMed 29462720