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Addgene

pRS416-BXG-altTAG
(Plasmid #158146)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158146 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS416
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4898
  • Total vector size (bp) 7371
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BFP-GFP fragment (w/ TAG codon)
  • Insert Size (bp)
    2500
  • Promoter GAL1-10

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AGTTAGCTCACTCATTAGGCACCC
  • 3′ sequencing primer GGATGTGCTGCAAGGCGATTA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS416-BXG-altTAG was a gift from James Van Deventer (Addgene plasmid # 158146 ; http://n2t.net/addgene:158146 ; RRID:Addgene_158146)

  • For your References section:

    Reporter system architecture affects measurements of noncanonical amino acid incorporation efficiency and fidelity. Potts KA, Stieglitz JT, Lei M, Van Deventer JA. Mol Syst Des Eng. 2020 Feb 1;5(2):573-588. doi: 10.1039/c9me00107g. Epub 2020 Jan 23. 10.1039/c9me00107g PubMed 33791108
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