FCK-ChR2-GFP
(Plasmid #15814)

• Purpose: Lentiviral expression of ChR2-GFP for neuronal excitation
• Depositing Lab: Edward Boyden
• Publication: Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Backbone

• Vector backbone: FCK(1.3)GW
• Backbone manufacturer: Pavel Osten
• Backbone size w/o insert (bp): 9500
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ChR2
• Alt name: Channelrhodopsin-2
• Species: C. reinhardtii
• Insert Size (bp): 1000
• Promoter: CaMKII
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PLEASE CONTACT ED BOYDEN () FOR DETAILS ON THIS
REAGENT AND FURTHER REAGENTS IN THIS LINE.