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Addgene

pCTCON2-BXG-altTAG2
(Plasmid #158134)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158134 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCTCON2-BYG
  • Backbone size w/o insert (bp) 4693
  • Total vector size (bp) 7193
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BFP-GFP fragment
  • Insert Size (bp)
    2500
  • Mutation
    Cloned in a TAG codon at the second serine residue of the linker sequence
  • Promoter GAL1-10

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTGCATAACCACTTTAACTAATACTTTC
  • 3′ sequencing primer GTAAAGTTGGTAACGGAACG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCTCON2-BXG-altTAG2 was a gift from James Van Deventer (Addgene plasmid # 158134 ; http://n2t.net/addgene:158134 ; RRID:Addgene_158134)

  • For your References section:

    Reporter system architecture affects measurements of noncanonical amino acid incorporation efficiency and fidelity. Potts KA, Stieglitz JT, Lei M, Van Deventer JA. Mol Syst Des Eng. 2020 Feb 1;5(2):573-588. doi: 10.1039/c9me00107g. Epub 2020 Jan 23. 10.1039/c9me00107g PubMed 33791108
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