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Addgene

pSpCas9_mouse_Adar1_ccB
(Plasmid #158122)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158122 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSpCas9(BB)-2A-Puro PX459
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 9175
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Adar1
  • gRNA/shRNA sequence
    CACCGGGCCTTCTTTGGACAGGAGA
  • Species
    M. musculus (mouse)
  • GenBank ID
    56417
  • Entrez Gene
    Adar (a.k.a. Adar1, Adar1p110, Adar1p150, DRADA, mZaADAR)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BBsI (not destroyed)
  • 3′ cloning site BBs I (not destroyed)
  • 5′ sequencing primer GACTATCATATGCTTACCGT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSpCas9_mouse_Adar1_ccB was a gift from Emanuela Felley-Bosco (Addgene plasmid # 158122 ; http://n2t.net/addgene:158122 ; RRID:Addgene_158122)
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