pSpCas9_human_ADAR1_ccB
(Plasmid
#158116)
-
Purposedeletion hADAR1
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 158116 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepSpCas9(BB)-2A-Puro PX459
-
Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 9175
-
Vector typeMammalian Expression
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameAdar1
-
gRNA/shRNA sequenceCACC G ACCCGGGACAATTCATTCCG
-
SpeciesH. sapiens (human)
-
GenBank ID103
-
Entrez GeneADAR (a.k.a. ADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSpCas9_human_ADAR1_ccB was a gift from Emanuela Felley-Bosco (Addgene plasmid # 158116 ; http://n2t.net/addgene:158116 ; RRID:Addgene_158116)