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Addgene

pCI-Puro_mouse_Adar2_Tv1
(Plasmid #158111)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158111 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCI-puro
  • Backbone size w/o insert (bp) 5224
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    adar2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    525
  • GenBank ID
    110532
  • Entrez Gene
    Adarb1 (a.k.a. 1700057H01Rik, Adar2, D10Bwg0447e, Red1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-Puro_mouse_Adar2_Tv1 was a gift from Emanuela Felley-Bosco (Addgene plasmid # 158111 ; http://n2t.net/addgene:158111 ; RRID:Addgene_158111)
  • For your References section:

    Double-Stranded RNA Structural Elements Holding the Key to Translational Regulation in Cancer: The Case of Editing in RNA-Binding Motif Protein 8A. Abukar A, Wipplinger M, Hariharan A, Sun S, Ronner M, Sculco M, Okonska A, Kresoja-Rakic J, Rehrauer H, Qi W, Beusechem VWV, Felley-Bosco E. Cells. 2021 Dec 15;10(12). pii: cells10123543. doi: 10.3390/cells10123543. 10.3390/cells10123543 PubMed 34944051