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pmirGlo-RBM8A-3'UTR 240-546
(Plasmid #158108)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158108 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmirGlo
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 7350
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    3'UTR of hRBM8A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    307
  • GenBank ID
    9939
  • Entrez Gene
    RBM8A (a.k.a. BOV-1A, BOV-1B, BOV-1C, C1DELq21.1, DEL1q21.1, MDS014, RBM8, RBM8B, TAR, Y14, ZNRP, ZRNP1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site Sal I (not destroyed)
  • 5′ sequencing primer not used
  • 3′ sequencing primer T7rem
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmirGlo-RBM8A-3'UTR 240-546 was a gift from Emanuela Felley-Bosco (Addgene plasmid # 158108 ; http://n2t.net/addgene:158108 ; RRID:Addgene_158108)

  • For your References section:

    Double-Stranded RNA Structural Elements Holding the Key to Translational Regulation in Cancer: The Case of Editing in RNA-Binding Motif Protein 8A. Abukar A, Wipplinger M, Hariharan A, Sun S, Ronner M, Sculco M, Okonska A, Kresoja-Rakic J, Rehrauer H, Qi W, Beusechem VWV, Felley-Bosco E. Cells. 2021 Dec 15;10(12). pii: cells10123543. doi: 10.3390/cells10123543. 10.3390/cells10123543 PubMed 34944051
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