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pEMS2075
(Plasmid #158098)

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Item Catalog # Description Quantity Price (USD)
Plasmid 158098 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    GenScript
  • Total vector size (bp) 3673

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    smCBA
  • Alt name
    Small chicken beta-actin promoter/cytomegalovirus enhancer
  • Insert Size (bp)
    947
  • Promoter smCBA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site FseI (not destroyed)
  • 5′ sequencing primer M13-fwd
  • 3′ sequencing primer M13-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid commercially produced by Genscript . Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid, including the MiniPromoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS2075 was a gift from Elizabeth Simpson (Addgene plasmid # 158098 ; http://n2t.net/addgene:158098 ; RRID:Addgene_158098)

  • For your References section:

    New MiniPromoter Ple389 (ADORA2A) drives selective expression in medium spiny neurons in mice and non-human primates. de Moura Gomes A, L Petkau T, J Korecki A, Fornes O, Galvan A, Lu G, M Hill A, Ling Lam S, Yao A, A Farkas R, W Wasserman W, Smith Y, M Simpson E, R Leavitt B. Sci Rep. 2024 Nov 15;14(1):28194. doi: 10.1038/s41598-024-79004-y. 10.1038/s41598-024-79004-y PubMed 39548191
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