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PurposeInducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158091 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUCM-CLYBL-hNIL
- Backbone size w/o insert (bp) 6069
- Total vector size (bp) 13176
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Vector typeMammalian Expression, TALEN
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Selectable markersGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCLYBL-CAG-DDdCas9VPH-T2A-GFP
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SpeciesSynthetic
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Insert Size (bp)7107
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site MluI (unknown if destroyed)
- 5′ sequencing primer CgccaccATGATCAGTCTGATTGCG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRT043-CLYBL-DDdcas9-VPH was a gift from Martin Kampmann (Addgene plasmid # 158091 ; http://n2t.net/addgene:158091 ; RRID:Addgene_158091) -
For your References section:
Genome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosis. Tian R, Abarientos A, Hong J, Hashemi SH, Yan R, Drager N, Leng K, Nalls MA, Singleton AB, Xu K, Faghri F, Kampmann M. Nat Neurosci. 2021 May 24. pii: 10.1038/s41593-021-00862-0. doi: 10.1038/s41593-021-00862-0. 10.1038/s41593-021-00862-0 PubMed 34031600
Map uploaded by the depositor.
