pET15-UbE2W
(Plasmid #15809)

• Depositing Lab: Wade Harper
• Publication: Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Backbone

• Vector backbone: pET15
• Backbone size w/o insert (bp): 5700
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ubiquitin conjugating enzyme UbE2W
• Alt name: UbE2W
• Species: H. sapiens (human)
• Insert Size (bp): 489
• GenBank ID: NM_001001481
• Entrez Gene: UBE2W (a.k.a. UBC-16, UBC16)
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC NGS analysis identifies a potential sequence discrepancy in the 3' end of the sequence encoding for LacI in the plasmid backbone. It is unknown what, if any, impact this may have on plasmid function.