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Addgene

pyEvolvR (enCas9-PolI5M)
(Plasmid #158073)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158073 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    custom
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    enCas9-PolI5M

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pyEvolvR (enCas9-PolI5M) was a gift from John Dueber (Addgene plasmid # 158073 ; http://n2t.net/addgene:158073 ; RRID:Addgene_158073)

  • For your References section:

    Targeted Diversification in the S. cerevisiae Genome with CRISPR-Guided DNA Polymerase I. Tou CJ, Schaffer DV, Dueber JE. ACS Synth Biol. 2020 Jun 16. doi: 10.1021/acssynbio.0c00149. 10.1021/acssynbio.0c00149 PubMed 32485105
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