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pTRE3G-EGFP-IRES(Neo)R
(Plasmid #158068)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158068 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRE3G-IRES
  • Backbone manufacturer
    Clontech Laboratories, Inc. A Takara Bio Compa
  • Backbone size w/o insert (bp) 4780
  • Total vector size (bp) 5500
  • Modifications to backbone
    1. Insertion of sequence encoding EGFP 2. Replacement of Amp resistance cassette with Neo/Kan resistance cassette
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP
  • Alt name
    GFP
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • GenBank ID
  • Promoter TRE3G
  • Tag / Fusion Protein
    • eGFP

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GCAGAGCTCGTTTAGTGAACC
  • 3′ sequencing primer GCCAAAAGACGGCAATATGGTGGA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE3G-EGFP-IRES(Neo)R was a gift from Adolfo Rivero-Muller (Addgene plasmid # 158068 ; http://n2t.net/addgene:158068 ; RRID:Addgene_158068)

  • For your References section:

    An Improved Vector System for Homogeneous and Stable Gene Regulation. Michalec-Wawiorka B, Czapinski J, Filipek K, Rulak P, Czerwonka A, Tchorzewski M, Rivero-Muller A. Int J Mol Sci. 2021 May 14;22(10). pii: ijms22105206. doi: 10.3390/ijms22105206. 10.3390/ijms22105206 PubMed 34069024
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