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Addgene

pdCas9-AsiA
(Plasmid #158063)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158063 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pdCas9-bacteria
  • Backbone size w/o insert (bp) 2500
  • Total vector size (bp) 7000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dCas9-AsiA
  • Species
    Synthetic
  • Insert Size (bp)
    4413
  • Promoter TetO

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACCTCATTAAGCAGCTCTAATGCGCTG
  • 3′ sequencing primer TGTGACTCTAGTAGAGAGCGTTCAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pdCas9-AsiA was a gift from Harris Wang (Addgene plasmid # 158063 ; http://n2t.net/addgene:158063 ; RRID:Addgene_158063)

  • For your References section:

    Programmable CRISPR-Cas transcriptional activation in bacteria. Ho HI, Fang JR, Cheung J, Wang HH. Mol Syst Biol. 2020 Jul;16(7):e9427. doi: 10.15252/msb.20199427. 10.15252/msb.20199427 PubMed 32657546
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