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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15805 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHMCV
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4839
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name10A1 Murine leukemia virus envelope mRNA
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SpeciesMoloney murine leukemia virus 10A1 strain
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Insert Size (bp)1938
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GenBank IDM33470
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHCMV-10A1 was a gift from Miguel Sena-Esteves (Addgene plasmid # 15805 ; http://n2t.net/addgene:15805 ; RRID:Addgene_15805) -
For your References section:
Optimized large-scale production of high titer lentivirus vector pseudotypes. Sena-Esteves M, Tebbets JC, Steffens S, Crombleholme T, Flake AW. J Virol Methods. 2004 Dec 15. 122(2):131-9. 10.1016/j.jviromet.2004.08.017 PubMed 15542136
Map uploaded by the depositor.
