pCMV-mGold-Calnexin-N-14
(Plasmid #158004)

• Purpose: Visualization of ER in mammalian cells using mGold
• Depositing Lab: Francois St-Pierre
• Publication: Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/eabb7438. doi: 10.1126/sciadv.abb7438. Print 2020 Oct.

Backbone

• Vector backbone: pCMV
• Total vector size (bp): 6497
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Calnexin
• Species: H. sapiens (human)
• Mutation: I474N
• GenBank ID: NM_001746.3
• Entrez Gene: CANX (a.k.a. CNX, IP90, P90)
• Promoter: CMV
• Tag / Fusion Protein:
  • mGold

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Existing fluorescent protein in plasmid Adggene# 56324 was replaced with mGold.
"N-14" of the name of the plasmid indicates the position of the tag (N- or C-terminal with respect to the fluorescent protein) and the number of residues in the linker between the two proteins. Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold.