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pCaggs-NLS-PAmCherry1-GSS-EGFP
(Plasmid #158000)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158000 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCaggs
  • Backbone size w/o insert (bp) 6105
  • Total vector size (bp) 7584
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS-PAmCherry1-GSS-EGFP
  • Species
    Synthetic
  • Insert Size (bp)
    1479
  • Promoter Actin promoter with CMV enhancer

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer aaggtggtggctggtgtggc
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PAmCherry1: FPBaseID OMODZ
EGFP: FPBaseID R9NL8

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCaggs-NLS-PAmCherry1-GSS-EGFP was a gift from Francois St-Pierre (Addgene plasmid # 158000 ; http://n2t.net/addgene:158000 ; RRID:Addgene_158000)

  • For your References section:

    Versatile phenotype-activated cell sorting. Lee J, Liu Z, Suzuki PH, Ahrens JF, Lai S, Lu X, Guan S, St-Pierre F. Sci Adv. 2020 Oct 23;6(43). pii: 6/43/eabb7438. doi: 10.1126/sciadv.abb7438. Print 2020 Oct. 10.1126/sciadv.abb7438 PubMed 33097540
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