pVL1393-MAL-SYK
(Plasmid
#157990)
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PurposeExpresses SYK in insect cell (sf9)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 157990 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepVL1393
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSpleen tyrosine kinase
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Alt nameSYK
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SpeciesH. sapiens (human)
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Entrez GeneSYK (a.k.a. IMD82, p72-Syk)
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Tags
/ Fusion Proteins
- Met-Ala-Leu (N terminal on insert)
- add Leu,Glu residues +His tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVL1393-MAL-SYK was a gift from Byung Il Lee (Addgene plasmid # 157990 ; http://n2t.net/addgene:157990 ; RRID:Addgene_157990) -
For your References section:
Crystal Structures of Spleen Tyrosine Kinase in Complex with Two Novel 4-Aminopyrido[4,3-d] Pyrimidine Derivative Inhibitors. Lee SJ, Choi JS, Bong SM, Hwang HJ, Lee J, Song HJ, Lee J, Kim JH, Koh JS, Lee BI. Mol Cells. 2018 Jun;41(6):545-552. doi: 10.14348/molcells.2018.2219. Epub 2018 Jun 12. 10.14348/molcells.2018.2219 PubMed 29890824
