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Addgene

pHis-Laforin
(Plasmid #157989)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157989 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHis vector(modified version of pET-28)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Laforin
  • Species
    H. sapiens (human)
  • Entrez Gene
    EPM2A (a.k.a. EPM2, MELF)
  • Tags / Fusion Proteins
    • His Tag (N terminal on insert)
    • His Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHis-Laforin was a gift from Byung Il Lee (Addgene plasmid # 157989 ; http://n2t.net/addgene:157989 ; RRID:Addgene_157989)

  • For your References section:

    Recombinant Human Laforin Expressed in Insect Cells: Expression, Purification, and Biochemical Characterizations. Choi J, Bong SM, Yang SW, Jang H, Park S, Kim SJ, Lee BI. J Kor Chem Soc, 2015; 59(5): 466-470. 10.5012/jkcs.2015.59.5.466
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