pCAG R2pH-LAMP1-3xFLAG
(Plasmid #157940)

• Purpose: expresses ratiometric sensor of lysosomal lumenal pH
• Depositing Lab: Massimiliano Stagi
• Publication: Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080/15548627.2020.1771858.

Backbone

• Vector backbone: pCAG
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: R2pH-LAMP1-3xFLAG
• Species: M. musculus (mouse)
• Insert Size (bp): 2745
• GenBank ID: NM_001317353.1:229-1452
• Entrez Gene: Lamp1 (a.k.a. CD107a, LGP-120, LGP-A, Lamp-1, P2B, Perk)
• Promoter: Chick beta actin pomoter
• Tags / Fusion Proteins:
  • mCherry
  • pHluorin
  • 3XFLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GCAACGTGCTGGTTATTGTG
• 3′ sequencing primer: CCAGAAGTCAGATGCTCAAGGGGTTT

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry