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pCD5-PR8-TEV-sfGFP-twinStrep
(Plasmid #157933)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157933 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCD5
  • Backbone manufacturer
    -
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 6500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MC1061
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    A/PuertoRico/8/34 hemagglutinin
  • Species
    Synthetic
  • Insert Size (bp)
    1500
  • Mutation
    E190D
  • Entrez Gene
    HA (a.k.a. FLUAVs4gp1)
  • Promoter CMV
  • Tag / Fusion Protein
    • sfGFP & TwinStrep (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer GGC TAA CTA GAG AAC CCA CTG C
  • 3′ sequencing primer TTC AGG GGG AGG TGT GGG AG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCD5-PR8-TEV-sfGFP-twinStrep was a gift from Robert de Vries (Addgene plasmid # 157933 ; http://n2t.net/addgene:157933 ; RRID:Addgene_157933)

  • For your References section:

    Fluorescent Trimeric Hemagglutinins Reveal Multivalent Receptor Binding Properties. Nemanichvili N, Tomris I, Turner HL, McBride R, Grant OC, van der Woude R, Aldosari MH, Pieters RJ, Woods RJ, Paulson JC, Boons GJ, Ward AB, Verheije MH, de Vries RP. J Mol Biol. 2019 Feb 15;431(4):842-856. doi: 10.1016/j.jmb.2018.12.014. Epub 2018 Dec 29. 10.1016/j.jmb.2018.12.014 PubMed 30597163
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