HA-Casp-2L
(Plasmid
#157932)
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PurposeOver expression of Casp-2L
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 157932 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)/myc-His A
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCasp2
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SpeciesM. musculus (mouse)
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Insert Size (bp)1400
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Entrez GeneCasp2 (a.k.a. CASP-2, ICH-1, NEDD-2, Nedd2)
- Promoter CMV
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Tags
/ Fusion Proteins
- HA (N terminal on insert)
- Myc (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7_promoter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA-Casp-2L was a gift from Baoji Xu (Addgene plasmid # 157932 ; http://n2t.net/addgene:157932 ; RRID:Addgene_157932) -
For your References section:
Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3beta signaling. Xu ZX, Tan JW, Xu H, Hill CJ, Ostrovskaya O, Martemyanov KA, Xu B. Nat Commun. 2019 Aug 9;10(1):3622. doi: 10.1038/s41467-019-11575-1. 10.1038/s41467-019-11575-1 PubMed 31399584