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Addgene

Rictor-Myc
(Plasmid #157931)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157931 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUltra
  • Backbone size w/o insert (bp) 8326
  • Total vector size (bp) 12700
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rictor
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    5127
  • GenBank ID
    NM_030168.3
  • Entrez Gene
    Rictor (a.k.a. 4921505C17Rik, 6030405M08Rik, AVO3, D530039E11Rik)
  • Promoter Ubiquitinc Promoter
  • Tags / Fusion Proteins
    • Myc (C terminal on insert)
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer hUBCpro-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rictor-Myc was a gift from Baoji Xu (Addgene plasmid # 157931 ; http://n2t.net/addgene:157931 ; RRID:Addgene_157931)

  • For your References section:

    Caspase-2 promotes AMPA receptor internalization and cognitive flexibility via mTORC2-AKT-GSK3beta signaling. Xu ZX, Tan JW, Xu H, Hill CJ, Ostrovskaya O, Martemyanov KA, Xu B. Nat Commun. 2019 Aug 9;10(1):3622. doi: 10.1038/s41467-019-11575-1. 10.1038/s41467-019-11575-1 PubMed 31399584
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