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Addgene

pHCMV-LCMV-WE
(Plasmid #15793)

Ordering

This material is available to academics and nonprofits only. Availability may be limited outside the U.S. Please log in for more information.
Item Catalog # Description Quantity Price (USD)
Plasmid 15793 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pHMCV
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4831
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lymphocytic choriomeningitis virus glycoprotein LCMVGP gene
  • Species
    Lymphocytic choriomeningitis virus (strain WE)
  • Insert Size (bp)
    1494
  • GenBank ID
    AF186080

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene was cloned by PCR from DNA isolated from cells infected with virus obtained from The Scripps Research Institute, La Jolla, CA
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHCMV-LCMV-WE was a gift from Miguel Sena-Esteves (Addgene plasmid # 15793 ; http://n2t.net/addgene:15793 ; RRID:Addgene_15793)
  • For your References section:

    Optimized large-scale production of high titer lentivirus vector pseudotypes. Sena-Esteves M, Tebbets JC, Steffens S, Crombleholme T, Flake AW. J Virol Methods. 2004 Dec 15. 122(2):131-9. 10.1016/j.jviromet.2004.08.017 PubMed 15542136