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Addgene

pPK-352
(Plasmid #157922)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157922 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression, Synthetic Biology ; Optogenetics

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    10-beta
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    tPcyA
  • Alt name
    Phycocyanobilin:ferredoxin oxidoreductase
  • Species
    S. cerevisiae (budding yeast); Thermosynechococcus elongatus
  • Insert Size (bp)
    799
  • Promoter CMV
  • Tag / Fusion Protein
    • Mitochondrial Targeting sequence (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XBA (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer SP6 promoter
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    IRES
  • Alt name
    Internal Ribosomal Entry Site
  • Insert Size (bp)
    627

Gene/Insert 3

  • Gene/Insert name
    HO1
  • Alt name
    Heme Oxygenase
  • Species
    Thermosynechococcus elongatus
  • Insert Size (bp)
    813
  • Tag / Fusion Protein
    • Mitochondrial Targeting Sequence (N terminal on insert)

Gene/Insert 4

  • Gene/Insert name
    FD
  • Alt name
    Ferredoxin
  • Species
    Thermosynechococcus elongatus
  • Insert Size (bp)
    477
  • Tag / Fusion Protein
    • Mitochondrial Targeting Sequence and V5 tag (N terminal on insert)

Gene/Insert 5

  • Gene/Insert name
    FNR
  • Alt name
    Ferredoxin-NADP reductase
  • Species
    Thermosynechococcus elongatus
  • Insert Size (bp)
    1290
  • Tag / Fusion Protein
    • Mitochondrial Targeting Sequence and HIS tag (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPK-352 was a gift from Phillip Kyriakakis (Addgene plasmid # 157922 ; http://n2t.net/addgene:157922 ; RRID:Addgene_157922)

  • For your References section:

    Building a Simple and Versatile Illumination System for Optogenetic Experiments. Kyriakakis P, Fernandez de Cossio L, Howard PW, Kouv S, Catanho M, Hu VJ, Kyriakakis R, Allen ME, Ma Y, Aguilar-Rivera M, Coleman TP. J Vis Exp. 2021 Jan 12;(167). doi: 10.3791/61914. 10.3791/61914 PubMed 33522514
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