pPK-351
(Plasmid #157921)

• Purpose: pcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBD
• Depositing Lab: Phillip Kyriakakis
• Publication: Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.3791/61914.

Backbone

• Vector backbone: pcDNA3.1
• Vector type: Mammalian Expression, Synthetic Biology ; Optogenetics

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Growth instructions: This plasmid can grow slowly and have low yields but is more stable than pPK-230.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: PIF3-MTAD
• Species: H. sapiens (human), A. thaliana (mustard weed)
• Insert Size (bp): 701
• Mutation: pif3 1-523
• Promoter: CMV
• Tag / Fusion Protein:
  • SV40NLS (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: T7 promoter

Gene/Insert 2

• Gene/Insert name: IRES
• Alt name: Internal Ribosomal Entry Site
• Insert Size (bp): 583

Gene/Insert 3

• Gene/Insert name: PhyB(1-621)-SV40NLS-Gal4DBD
• Species: S. cerevisiae (budding yeast), A. thaliana (mustard weed)
• Insert Size (bp): 2391
• Tag / Fusion Protein:
  • HA tag (C terminal on insert)

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: GACGTGGTTTTCCTTTGAAAAACAC
• 3′ sequencing primer: SP6 promoter

Resource Information

• Supplemental Documents:
  • 351 - pcDNA-CMV-PIF3-MTAD-IRES-PhyB-G4DBD.gb
  • 351 - pcDNA-CMV-PIF3-MTAD-IRES-PhyB-G4DBD.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry