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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15791 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST17
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameUbiquitin conjugating enzyme UbE2G2
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Alt nameUbE2G2
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SpeciesH. sapiens (human)
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Insert Size (bp)498
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GenBank IDNM_003343
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Entrez GeneUBE2G2 (a.k.a. UBC7)
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST17-UbE2G2 was a gift from Wade Harper (Addgene plasmid # 15791 ; http://n2t.net/addgene:15791 ; RRID:Addgene_15791) -
For your References section:
Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Jin J, Li X, Gygi SP, Harper JW. Nature. 2007 Jun 28. 447(7148):1135-8. 10.1038/nature05902 PubMed 17597759