pLG007 (Ec86 retron)
(Plasmid
#157885)
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PurposeExpresses the Ec86 retron defense system
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 157885 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACYC184
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEc86 retron
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SpeciesEscherichia coli BL21
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Insert Size (bp)2188
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLG007 (Ec86 retron) was a gift from Feng Zhang (Addgene plasmid # 157885 ; http://n2t.net/addgene:157885 ; RRID:Addgene_157885) -
For your References section:
Diverse enzymatic activities mediate antiviral immunity in prokaryotes. Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk JL, Koob J, Wolf YI, Koonin EV, Zhang F. Science. 2020 Aug 28;369(6507):1077-1084. doi: 10.1126/science.aba0372. 10.1126/science.aba0372 PubMed 32855333