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Addgene

SARS-CoV-2_N_deltaCIDR
(Plasmid #157872)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157872 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTHMT
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N
  • Species
    SARS-CoV-2
  • Mutation
    deltaCIDR
  • Entrez Gene
    N (a.k.a. GU280_gp10)
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains a D15H mutation in MBP. This mutation is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SARS-CoV-2_N_deltaCIDR was a gift from Nicolas Fawzi (Addgene plasmid # 157872 ; http://n2t.net/addgene:157872 ; RRID:Addgene_157872)

  • For your References section:

    SARS-CoV-2 nucleocapsid protein phase-separates with RNA and with human hnRNPs. Perdikari TM, Murthy AC, Ryan VH, Watters S, Naik MT, Fawzi NL. EMBO J. 2020 Nov 17:e106478. doi: 10.15252/embj.2020106478. 10.15252/embj.2020106478 PubMed 33200826
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