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Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 157867 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTHMT
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameN
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SpeciesSARS-CoV-2
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Entrez GeneN (a.k.a. GU280_gp10)
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Tag
/ Fusion Protein
- MBP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SARS-CoV-2_N_FL was a gift from Nicolas Fawzi (Addgene plasmid # 157867 ; http://n2t.net/addgene:157867 ; RRID:Addgene_157867) -
For your References section:
SARS-CoV-2 nucleocapsid protein phase-separates with RNA and with human hnRNPs. Perdikari TM, Murthy AC, Ryan VH, Watters S, Naik MT, Fawzi NL. EMBO J. 2020 Nov 17:e106478. doi: 10.15252/embj.2020106478. 10.15252/embj.2020106478 PubMed 33200826