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Addgene

pAtetO_6xHis-Klentaq
(Plasmid #157862)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157862 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAtetO
  • Backbone manufacturer
    modified from pASK-IBA37plus (IBA GmbH)
  • Modifications to backbone
    Removal of 6xHis tag, multiple cloning site, and Rop gene from pASK-IBA37plus
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Klentaq
  • Alt name
    Klentaq polymerase
  • Species
    Thermus aquaticus
  • Insert Size (bp)
    1710
  • Promoter tet PA
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTTGTTTAACTTTAAGAAGGAGATATACATATGGCTAGCAGAGGATCGCATCACCATCACCATCACATCGAAGGGCGCCTTCTTCACGAGTTCGGAC
  • 3′ sequencing primer GAAGATTGGTTATCTGCGAAAGAATGATAATTGCCTCTGCCAAAATTCTGTCCTCAAGCG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is an N82D mismatch in the TetR coding sequence that is not known to be of functional concern.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAtetO_6xHis-Klentaq was a gift from Andrew Ellington (Addgene plasmid # 157862 ; http://n2t.net/addgene:157862 ; RRID:Addgene_157862)

  • For your References section:

    Evolution of a Thermophilic Strand-Displacing Polymerase Using High-Temperature Isothermal Compartmentalized Self-Replication. Milligan JN, Shroff R, Garry DJ, Ellington AD. Biochemistry. 2018 Aug 7;57(31):4607-4619. doi: 10.1021/acs.biochem.8b00200. Epub 2018 Apr 18. 10.1021/acs.biochem.8b00200 PubMed 29629759
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