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Addgene

FMR1-E17B-P2A-NLuc
(Plasmid #157857)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157857 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Addgene #31938
  • Backbone size w/o insert (bp) 8012
  • Total vector size (bp) 6616
  • Modifications to backbone
    replacing OCT4 homology arms and inserts with FMR1 homology arms and P2A-Nluc
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FMR1-Nlu
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2700
  • Entrez Gene
    FMR1 (a.k.a. FMRP, FRAXA, POF, POF1)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FMR1-E17B-P2A-NLuc was a gift from Xinyu Zhao (Addgene plasmid # 157857 ; http://n2t.net/addgene:157857 ; RRID:Addgene_157857)

  • For your References section:

    Establishment of Reporter Lines for Detecting Fragile X Mental Retardation (FMR1) Gene Reactivation in Human Neural Cells. Li M, Zhao H, Ananiev GE, Musser MT, Ness KH, Maglaque DL, Saha K, Bhattacharyya A, Zhao X. Stem Cells. 2017 Jan;35(1):158-169. doi: 10.1002/stem.2463. Epub 2016 Jul 27. 10.1002/stem.2463 PubMed 27422057
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