pDEST17-UbE2D3
(Plasmid #15784)

• Depositing Lab: Wade Harper
• Publication: Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Backbone

• Vector backbone: pDEST17
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Ubiquitin conjugating enzyme UbE2D3
• Alt name: UbE2D3
• Species: H. sapiens (human)
• Insert Size (bp): 450
• Mutation: see Depositor Comments below
• GenBank ID:
• Entrez Gene: UBE2D3 (a.k.a. E2(17)KB3, UBC4/5, UBCH5C)
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC NGS analysis identifies a P18L discrepancy relative to NP_003331.1. It is unknown what, if any, impact this may have on plasmid function.