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Addgene

G1333 DddAtox-C–dSpCas9
(Plasmid #157834)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157834 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Broad Institute and the University of Washington have filed provisional patent applications on base-editing systems described in this study, listing B.Y.M., M.H.d.M., S.B.P., J.D.M. and D.R.L. as inventors

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    G1333 DddAtox-C–dSpCas9 was a gift from David Liu (Addgene plasmid # 157834 ; http://n2t.net/addgene:157834 ; RRID:Addgene_157834)

  • For your References section:

    A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Mok BY, de Moraes MH, Zeng J, Bosch DE, Kotrys AV, Raguram A, Hsu F, Radey MC, Peterson SB, Mootha VK, Mougous JD, Liu DR. Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4. 10.1038/s41586-020-2477-4 PubMed 32641830
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