pET15-UbE2D1
(Plasmid #15782)

• Depositing Lab: Wade Harper
• Publication: Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Backbone

• Vector backbone: pET15 modified
• Backbone size w/o insert (bp): 5700
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ubiquitin conjugating enzyme UbE2D1
• Alt name: UbE2D1
• Species: H. sapiens (human)
• Insert Size (bp): 444
• GenBank ID: NM_003338
• Entrez Gene: UBE2D1 (a.k.a. E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A)
• Tags / Fusion Proteins:
  • 6x His (N terminal on backbone)
  • T7 tag (N terminal on backbone)
  • Xpress tag (enterokinase cleavage site) (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry